Effect of stage 0 sporulation mutations on subtilisin expression.

@article{Ferrari1986EffectOS,
  title={Effect of stage 0 sporulation mutations on subtilisin expression.},
  author={Eug{\^e}nio Alvarenga Ferrari and Sandra M. H. Howard and James Alfred Hoch},
  journal={Journal of bacteriology},
  year={1986},
  volume={166 1},
  pages={
          173-9
        }
}
Subtilisin expression as a function of growth and sporulation was determined using a presubtilisin-beta-galactosidase gene fusion. An approximately 500-base-pair region upstream of the subtilisin gene and including the first eight codons of the presubtilisin protein was fused at the eighth codon of beta-galactosidase in the integrative vector pJF751. This gene fusion does not carry a signal sequence, and therefore its synthesis is uncoupled from maturation of presubtilisin. The fusion protein… CONTINUE READING
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