Effect of sperm treatment on efficiency of EGFP-expressing porcine embryos produced by ICSI-SMGT.

@article{GarcaVzquez2009EffectOS,
  title={Effect of sperm treatment on efficiency of EGFP-expressing porcine embryos produced by ICSI-SMGT.},
  author={F. Garc{\'i}a-V{\'a}zquez and E. Garc{\'i}a-Rosell{\'o} and A. Guti{\'e}rrez-Ad{\'a}n and J. Gadea},
  journal={Theriogenology},
  year={2009},
  volume={72 4},
  pages={
          506-18
        }
}
  • F. García-Vázquez, E. García-Roselló, +1 author J. Gadea
  • Published 2009
  • Biology, Medicine
  • Theriogenology
  • Intracytoplasmic sperm injection-sperm-mediated gene transfer (ICSI-SMGT) is a useful tool for the production of transgenic mice but is still rather inefficient in farm animals. In the current study, we evaluated the effect of the sperm treatments on the efficiency for producing enhanced green fluorescent protein (EGFP)-expressing pig embryos by ICSI-SMGT. Four different sperm treatments were assayed: (1) fresh (control), (2) frozen-thawing (FT), (3) quick freezing without cryoprotectant agents… CONTINUE READING
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