Effect of sperm preparation method on in vitro fertilization in pigs.

@article{Mats2003EffectOS,
  title={Effect of sperm preparation method on in vitro fertilization in pigs.},
  author={Carmen Mat{\'a}s and Pilar Coy and Raquel Romar and Montanari Marco and Joaqu{\'i}n Gadea and Salvador Ruiz},
  journal={Reproduction},
  year={2003},
  volume={125 1},
  pages={133-41}
}
This study was designed to determine the effect of different sperm preparation treatments before IVF on the acrosome reaction, oocyte penetration time, early embryo development and timing of female and male pronucleus formation. Pooled sperm-rich fractions were (i) washed in PBS, (ii) left unwashed, or (iii) layered in a Percoll gradient. In Expt 1, the proportion of acrosome-reacted spermatozoa, determined by staining with fluorescein isothyocyanate-labelled peanut agglutinin lectin and… CONTINUE READING