Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria

@article{Hiltner1983EffectOS,
  title={Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria},
  author={P. Hiltner and B. A. Dehority},
  journal={Applied and Environmental Microbiology},
  year={1983},
  volume={46},
  pages={642 - 648}
}
The rate of cellulose digestion in the presence of either glucose or cellobiose was studied for the three predominant species of cellulolytic rumen bacteria: Ruminococcus albus, Ruminococcus flavefaciens, and Bacteroides succinogenes. When a soluble carbohydrate was added to cellulose broth, the lag phase of cellulose digestion was shortened. Presumably, this was due to greater numbers of bacteria, because increasing the size of the inoculum had a similar effect. Cellulose digestion occurred… Expand
Cellobiose versus glucose utilization by the ruminal bacterium Ruminococcus albus
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When the ruminal bacterium Ruminococcus albus was incubated with a combination ofcellobiose and glucose, the organism preferentially utilized the disaccharide, and this preference appeared to be related to repression of glucose uptake systems in cellobiose-grown cells. Expand
Quantitative analysis of cellulose degradation and growth of cellulolytic bacteria in the rumen.
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A dynamic model based on STELLA software is presented that evaluates all of the major aspects of ruminal cellulose degradation: ingestion, digestion and passage of feed particles, maintenance and growth of cellulolytic bacteria and pH effects. Expand
Response surface analysis of the effects of pH and dilution rate on Ruminococcus flavefaciens FD-1 in cellulose-fed continuous culture
  • Y. Shi, P. Weimer
  • Medicine, Chemistry
  • Applied and environmental microbiology
  • 1992
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The response surfaces indicate that pH and D coordinately affect cellulose digestion and growth yield in this organism and the reduced cell yield at low pH appears to be due to both an increase in maintenance energy requirements and a decrease in true growth yield. Expand
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Data support the contention that the protrusions have a role in cellulose hydrolysis; however, this interpretation is complicated by reactivity of the antibodies with a large number of other proteins that possess related antigenic epitopes. Expand
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Based on reported concentrations of Ca(+2) in the rumen, it seems unlikely that an in vivo deficiency would occur for these bacteria, and the role of ionized calcium in growth and cellulose digestion is evaluated. Expand
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Pectin supplementation resulted in a lower proportion of residual sugars remaining from cell walls after 60 and 72 h, and the lack of differences in bacterial enzy- matic activities suggests the absence of qualitative or quantitative differences in the adherent fibrolytic population. Expand
Magnesium requirement of some of the principal rumen cellulolytic bacteria.
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Both strains of R. flavefaciens were found to have an absolute Mg requirement for cellulose degradation, and it seems unlikely that an in vivo deficiency of this element would occur. Expand
Kinetics of Cellulose Digestion by Fibrobacter succinogenes S85
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Growing cultures of Fibrobacter succinogenes S85 digested cellulose at a rapid rate, but nongrowing cells and cell extracts did not have detectable crystalline cellulase activity, and cells that were preincubated for a short time with thiocellobiose lost their ability to digest cellulose. Expand
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The rumen system provides an interesting case study in the context of possible process concepts for direct fermentation of biomass to commercially important chemicals such as acetate, propionate, succinate, lactate and ethanol. Expand
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