Effect of single amino acid replacements on the thermal stability of the NH 2-terminal domain of phage X repressor ( scanning calorimetry / circular dichroism / proteolysis / temperature-sensitive mutants )

The thermal stabilities of mutant phage X repressors that have single amino acid replacements in the NH2terminal domain have been studied by means of circular dichroism and differential scanning calorimetry. The variations in stability determined by these physical methods correlate with the resistance to proteolysis at various temperatures and can be… CONTINUE READING