Effect of short- or long-term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture

  title={Effect of short- or long-term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture},
  author={Joachim Wever and Dietrich O. Schachtschabel and Gerhard Sluke and Gudrun Wever},
  journal={Mechanisms of Ageing and Development},
[Decreased stimulation of glycosaminoglycan synthesis by human skin fibroblasts by interleukin 6 within the scope of in vitro aging].
The diminished response of GAG- and hyaluronic acid synthesis during aging of these in vitro cultured fibroblasts should motivate further research if similar processes occur during aging in an organism.
Cell surface components and growth regulation in cultivated arterial smooth muscle cells.
A role for cell-surface and pericellular glycoconjugates in growth regulation is suggested and these molecules, due to their anionic charge or by steric exclusion, interfere with the binding of platelet-derived growth factor, a highly cationic polypeptide, to its cell- surface receptor.
Effects of Hydrocortisone and Heparin on Growth and Glycosaminoglycan Synthesis of Cultured Human Fibroblasts (WI-38)
In contrast to “nonaging” tumor cells with unlimited proliferation potential in vitro (cell culture), normal diploid fibroblasts and epithelial cells undergo a limited number of divisions in culture,
Synthesis and composition of glycosaminoglycans by cultured human trabecular meshwork cells
Long-term cultivation under nondividing (“senescent”) conditions resulted in a change of the pattern of synthesized and excreted (medium) [14C]-glucosamine-labeled glycosaminoglycans with a relative decrease of hyaluronic acid and a relative increase of heparan sulfate.
In Vitro Studies of Fibroblast Aging: Growth Factors, Collagen and Glycosaminoglycan Synthesis — a Review
If one applies rigorous culture conditions with defined culture media, the findings of Carrel and Ebeling cannot be reproduced, and it was shown that chick fibroblasts have a limited life span of about 23 population doublings.
Heparan sulfate enhances growth of transformed human cells.
In this study the effects of HS on proliferation of transformed cells were studied in vitro and HS was found to affect cell growth not by modulation of growth-related substances in the medium, but through contact with the cell surface.
Effect of Heparin on Production of Basic Fibroblast Growth Factor and Transforming Growth Factor-Beta1 by Human Normal Skin and Hyperplastic Scar Fibroblasts
The effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastics scar formation and wound healing in vivo.


Influence of exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of cultured human diploid fibroblasts (WI-38).
Following long-term treatment with heparin, incorporation rates of both 14C-glucosamine and 35S-sulfate into both cell-bound and cell-released (medium) glycosaminoglycans were elevated, suggesting a general stimulation of glycosaminationoglycan synthesis.
Changes of glycosaminoglycan synthesis during in vitro ageing of human fibroblasts (WI-38).
The changes in glycosaminoglycan metabolism are interpreted as an expression of cellular ageing, and an in vitro system offers a model for analyzing the factors involved in or causing the induction respectively prevention of this functional change.
A comparative study of glycosaminoglycans in cultures of human, normal and malignant glial cells
The glycosaminoglycans of human cultured normal glial and malignant glioma cell lines were studied using 35S‐sulphate or 3H‐glucosamine as markers and release of newly synthesized GAG to the extracellular medium was a rapid process in the normal cells but was more or less delayed in theglioma cells.
Stimulation of chondromucoprotein synthesis in chondrocytes by extracellular chondromucoprotein.
  • Z. NevoA. Dorfman
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1972
It is suggested that the rate of chondromucoprotein synthesis may be partially controlled by cell surface receptors and incubation of cells after trypsinization results in restoration of the rates ofChondrocytes in culture.
Metabolic parameters of the growth-stimulating effect of chondroitin sulfate a in tissue cultures.∗
The demonstrated action of small amounts of chondroitin sulfate A in several types of cells, together with the finding that this material is normally present in human plasma in such amounts suggests that it may be a metabolic agent in the regulation of cell processes.
Growth-promoting activity of acid mucopolysaccharides on a strain of human mammary carcinoma cells.
It is suggested that the action of acid mucopolysaccharides on BT 20 cells is not of a nutritional, but of a physical or physico-chemical, nature and is exerted at the cell membrane.