Effect of short- or long-term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture

@article{Wever1980EffectOS,
  title={Effect of short- or long-term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture},
  author={J. Wever and D. Schachtschabel and G. Sluke and G. Wever},
  journal={Mechanisms of Ageing and Development},
  year={1980},
  volume={14},
  pages={89-99}
}
Short-term (several days) or long-term (several weeks and months) treatment of cultured human diploid fibroblasts (WI-38; phase II) with heparin at 20--500 micrograms/ml inhibited cell proliferation and stimulated glycosaminoglycan synthesis (as measured by the incorporation rates of [35S] sulfate and [14C] glucosamine into cellular and medium glycosaminoglycans). Characterization of the individual glycosaminoglycan types revealed an increased portion of incorporated radioactivity in the… Expand
Exogenous glycosaminoglycans stimulate hyaluronic acid synthesis by cultured human trabecular-meshwork cells.
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Age-related changes in the distribution pattern of glycosaminoglycans synthesized by cultured human diploid fibroblasts (WI-38)
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Long-term cultivation under nondividing (“senescent”) conditions resulted in a change of the pattern of synthesized and excreted (medium) [14C]-glucosamine-labeled glycosaminoglycans with a relative decrease of hyaluronic acid and a relative increase of heparan sulfate. Expand
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TLDR
Following long-term treatment with heparin, incorporation rates of both 14C-glucosamine and 35S-sulfate into both cell-bound and cell-released (medium) glycosaminoglycans were elevated, suggesting a general stimulation of glycosaminationoglycan synthesis. Expand
Changes of glycosaminoglycan synthesis during in vitro ageing of human fibroblasts (WI-38).
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The changes in glycosaminoglycan metabolism are interpreted as an expression of cellular ageing, and an in vitro system offers a model for analyzing the factors involved in or causing the induction respectively prevention of this functional change. Expand
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It is shown that hyaluronic acid inhibits chondroitin sulfate synthesis and the accumulation of newly synthesized proteoglycan in the cell layer, indicating that specific glycosaminoglycans, such as hyaluonic acid, can affect the synthesis and accumulation of other glycosaminecans and should be considered as possible regulators of specific cellular activities. Expand
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It is suggested that the gel-like properties of hyaluronic acid and the ion-binding capacity of the sulfated glycosaminoglycans might have a function in the filtering mechanism of the aqueous humor outflow. Expand
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In this system, only sulfated polyanions like those produced by the corneal epithelium have a stimulatory effect on glycosaminoglycan synthesis, and the results are discussed in terms of how the tissues of the cornea may interact by changing the composition of the stromal extracellular matrix. Expand
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The glycosaminoglycans of human cultured normal glial and malignant glioma cell lines were studied using 35S‐sulphate or 3H‐glucosamine as markers and release of newly synthesized GAG to the extracellular medium was a rapid process in the normal cells but was more or less delayed in theglioma cells. Expand
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