Effect of short- or long-term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture

@article{Wever1980EffectOS,
  title={Effect of short- or long-term treatment with exogenous glycosaminoglycans on growth and glycosaminoglycan synthesis of human fibroblasts (WI-38) in culture},
  author={Joachim Wever and Dietrich O. Schachtschabel and Gerhard Sluke and Gudrun Wever},
  journal={Mechanisms of Ageing and Development},
  year={1980},
  volume={14},
  pages={89-99}
}
[Decreased stimulation of glycosaminoglycan synthesis by human skin fibroblasts by interleukin 6 within the scope of in vitro aging].
TLDR
The diminished response of GAG- and hyaluronic acid synthesis during aging of these in vitro cultured fibroblasts should motivate further research if similar processes occur during aging in an organism.
Cell surface components and growth regulation in cultivated arterial smooth muscle cells.
TLDR
A role for cell-surface and pericellular glycoconjugates in growth regulation is suggested and these molecules, due to their anionic charge or by steric exclusion, interfere with the binding of platelet-derived growth factor, a highly cationic polypeptide, to its cell- surface receptor.
Effects of Hydrocortisone and Heparin on Growth and Glycosaminoglycan Synthesis of Cultured Human Fibroblasts (WI-38)
In contrast to “nonaging” tumor cells with unlimited proliferation potential in vitro (cell culture), normal diploid fibroblasts and epithelial cells undergo a limited number of divisions in culture,
Synthesis and composition of glycosaminoglycans by cultured human trabecular meshwork cells
TLDR
Long-term cultivation under nondividing (“senescent”) conditions resulted in a change of the pattern of synthesized and excreted (medium) [14C]-glucosamine-labeled glycosaminoglycans with a relative decrease of hyaluronic acid and a relative increase of heparan sulfate.
In Vitro Studies of Fibroblast Aging: Growth Factors, Collagen and Glycosaminoglycan Synthesis — a Review
TLDR
If one applies rigorous culture conditions with defined culture media, the findings of Carrel and Ebeling cannot be reproduced, and it was shown that chick fibroblasts have a limited life span of about 23 population doublings.
Heparan sulfate enhances growth of transformed human cells.
TLDR
In this study the effects of HS on proliferation of transformed cells were studied in vitro and HS was found to affect cell growth not by modulation of growth-related substances in the medium, but through contact with the cell surface.
Effect of Heparin on Production of Basic Fibroblast Growth Factor and Transforming Growth Factor-Beta1 by Human Normal Skin and Hyperplastic Scar Fibroblasts
TLDR
The effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastics scar formation and wound healing in vivo.
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TLDR
Following long-term treatment with heparin, incorporation rates of both 14C-glucosamine and 35S-sulfate into both cell-bound and cell-released (medium) glycosaminoglycans were elevated, suggesting a general stimulation of glycosaminationoglycan synthesis.
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TLDR
The changes in glycosaminoglycan metabolism are interpreted as an expression of cellular ageing, and an in vitro system offers a model for analyzing the factors involved in or causing the induction respectively prevention of this functional change.
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TLDR
The glycosaminoglycans of human cultured normal glial and malignant glioma cell lines were studied using 35S‐sulphate or 3H‐glucosamine as markers and release of newly synthesized GAG to the extracellular medium was a rapid process in the normal cells but was more or less delayed in theglioma cells.
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    Proceedings of the National Academy of Sciences of the United States of America
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TLDR
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TLDR
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TLDR
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