Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp. paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture.

@article{Pribylova2012EffectOS,
  title={Effect of short- and long-term antibiotic exposure on the viability of Mycobacterium avium subsp. paratuberculosis as measured by propidium monoazide F57 real time quantitative PCR and culture.},
  author={Radka Pribylova and Lucie Kubickova and Vladimir Babak and Ivo Pavl{\'i}k and Petr Kralik},
  journal={Veterinary journal},
  year={2012},
  volume={194 3},
  pages={354-60}
}
Mycobacterium avium subsp. paratuberculosis (MAP), the causative agent of paratuberculosis in ruminants, has a lipid-rich cell wall which facilitates its survival and persistence in the environment. This property of the organism is exploited when it is cultured as decontaminating agents and antibiotics are used to suppress the growth of contaminating microflora, but such treatments can also negatively affect the isolation of MAP itself. The objective of this study was to assess the effect of… CONTINUE READING