Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12.

  title={Effect of rfaH (sfrB) and temperature on expression of rfa genes of Escherichia coli K-12.},
  author={E L Pradel and Carl A. Schnaitman},
  journal={Journal of bacteriology},
  volume={173 20},
In order to study the regulation of a large block of contiguous genes at the rfa locus of Escherichia coli K-12 which are involved in synthesis and modification of the lipopolysaccharide core, the transposon TnlacZ was used to generate in-frame lacZ fusions to the coding regions of five genes (rfaQ, -G, -P, -B and -J) within this block. The beta-galactosidase activity of strains in which these fusions had been crossed into the chromosomal rfa locus was significantly decreased when the rfaH11… CONTINUE READING
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