Effect of reversible ATP depletion on tight-junction integrity in LLC-PK1 cells.

@article{Canfield1991EffectOR,
  title={Effect of reversible ATP depletion on tight-junction integrity in LLC-PK1 cells.},
  author={P E Canfield and A M Geerdes and Bruce A Molitoris},
  journal={The American journal of physiology},
  year={1991},
  volume={261 6 Pt 2},
  pages={F1038-45}
}
To further understand and investigate how ischemia affects the tight junction we have developed a 2-h model of rapidly reversible ATP depletion and cellular injury in confluent LLC-PK1 monolayers. ATP depletion was achieved utilizing substrate-free medium containing 0.1 microM antimycin A (AA). Cellular ATP levels dropped rapidly to less than 5% of control values, but recovery of ATP and cell morphology was possible even after 2 h of exposure to AA. Ruthenium red, an electron-dense marker of… CONTINUE READING
51 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 51 extracted citations

Similar Papers

Loading similar papers…