Effect of repeated freezing–thawing on the Achilles tendon of rabbits

@article{Chen2010EffectOR,
  title={Effect of repeated freezing–thawing on the Achilles tendon of rabbits},
  author={Lianxu Chen and Yanping Wu and Jia-Kuo Yu and Zhaode Jiao and Yingfang Ao and Chang-long Yu and Jian-Quan Wang and Guo-qing Cui},
  journal={Knee Surgery, Sports Traumatology, Arthroscopy},
  year={2010},
  volume={19},
  pages={1028-1034}
}
The increased use of allograft tissue in the reconstruction of anterior cruciate ligament has brought more focus to the effect of storage and treatment on allograft. The purpose of this study was to observe the effect of histology and biomechanics on Achilles tendon in rabbits through repeated freezing–thawing before allograft tendon transplantation. Rabbit Achilles tendons were harvested and processed according to the manufacture’s protocol of tissue bank, and freezing–thawing was repeated… Expand
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References

SHOWING 1-10 OF 29 REFERENCES
Effects of freezing on the biomechanical and structural properties of human posterior tibial tendons
TLDR
A high variability exists in the biophysical properties of tendons among individuals and in the effects of storage on tendons, Therefore, when choosing an allograft tendon, particular care is needed to choose a biomechanically suitable graft. Expand
The effects of in situ freezing on the anterior cruciate ligament. An experimental study in goats.
TLDR
It is concluded that devitalized, devascularized anterior cruciate ligaments do not lose strength if the anatomical position and the orientation of the collagen fibers are not altered. Expand
Hamstring tendon grafts for reconstruction of the anterior cruciate ligament: biomechanical evaluation of the use of multiple strands and tensioning techniques.
TLDR
The data demonstrate that equally tensioned four-strand hamstring-tendon grafts have initial tensile properties that are higher than those reported for ten-millimeter patellar-ligament grafts; thus, from a biomechanical point of view, they seem to be a reasonable alternative. Expand
Effects of postmortem storage by freezing on ligament tensile behavior.
The purpose of this study is to examine the effect of prolonged postmortem freezing storage (between 1 1/2 and 3 months at -20 degrees C) on the structural properties of the medial collateralExpand
Achilles tendon allograft reconstruction of the anterior cruciate ligament-deficient knee
TLDR
Reconstruction of the anterior cruciate ligament with intraarticular fresh-frozen Achilles tendon allograft and extraarticular tibial band tenodesis and results of clinical and functional examinations were favorable, but radiologic results were not; nevertheless, unexplained tunnel enlargement is cause for concern. Expand
Reconstruction of anterior cruciate ligaments with bone-patellar tendon-bone and achilles tendon allografts.
TLDR
It was concluded that either frozen or freeze-dried aseptically excised and processed bone-patellar tendon-bone and Achilles tendon allografts can serve adequately for the reconstruction of ACLs. Expand
Multiple freeze‐thaw cycled meniscal allograft tissue: A biomechanical, biochemical, and histologic analysis
TLDR
From results, transplantation of meniscal allograft tissue frozen and thawed four times may be compromised in its ability to resist compression; and thus may undermine its role in replacing native meniscal tissue. Expand
Replacement of the anterior cruciate ligament using a patellar tendon allograft. An experimental study.
TLDR
The anterior cruciate ligament of twenty-five adult dogs was replaced using fresh or deep-frozen patellar-tendon allografts using a vascular-injection technique at various intervals from two weeks to one year postoperatively to evaluate the morphology and gross and histological appearance. Expand
Anterior Cruciate Ligament Reconstruction Using Cryopreserved Allografts
TLDR
This experience shows that favorable results can be obtained with cryopreserved Achilles tendon allografts in athletes in whom avoiding donor site morbidity may be an issue in terms of a prompt return to sport. Expand
Irradiated meniscal allotransplants of rabbits: study of the mechanical properties at six months postoperation.
TLDR
Comparison of the long-term effects of gamma irradiation on the indentation properties of rabbits' meniscal allotransplants showed that compliance to long- term creep of the frozen irradiated menisci was significantly reduced, suggesting that at 6 months postoperatively, the Frozen irradiated allotransPlants were less viscous compared to fresh or frozen transplants. Expand
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