Effect of reduction and heat on the detection of house dust mite (Dermatophagoides pteronyssinus) allergen Der p I by protein blotting.

Abstract

Employment of reducing conditions during sample preparation alters the mobility of the house dust mite (Dermatophagoides pteronyssinus) allergen Der p I as determined by sodium dodecylsulphate-poly-acrylamide gel electrophoresis (SDS-PAGE) from an apparent MW of approximately 25 kD (unreduced) to an apparent MW of approximately 30 kD. Probing of… (More)

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Cite this paper

@article{Ford1989EffectOR, title={Effect of reduction and heat on the detection of house dust mite (Dermatophagoides pteronyssinus) allergen Der p I by protein blotting.}, author={S. A. Ford and Euan Roger Tovey and Barbara Baldo}, journal={International archives of allergy and applied immunology}, year={1989}, volume={89 2-3}, pages={318-20} }