Effect of reaction temperature on the determination of rat serum IgE by the avidin-biotin method.

@article{Yamada1996EffectOR,
  title={Effect of reaction temperature on the determination of rat serum IgE by the avidin-biotin method.},
  author={Ken-ichi Yamada and Akimasa Nakao and Mitsuo Takasugi and Yoshifumi Itoh and Nahida A Hassan and M. Sugano},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1996},
  volume={60 11},
  pages={1891-2}
}
We established an avidin-biotin method for the sensitive determination of rat IgE and found that a non-specific signal was generated depending on the reaction temperature. When the sera of rats immunized or not with ovalbumin (OVA) were fractionated in a hydroxyapatite column and OVA-specific IgE was determined by the avidin-biotin method at 4 degrees C or… CONTINUE READING