Effect of protonmotive force on the relative proton stoichiometries of the mitochondrial proton pumps.

@article{Hafner1991EffectOP,
  title={Effect of protonmotive force on the relative proton stoichiometries of the mitochondrial proton pumps.},
  author={Renate Hafner and Martin D. Brand},
  journal={The Biochemical journal},
  year={1991},
  volume={275 ( Pt 1)},
  pages={
          75-80
        }
}
The rate of phosphorylation of ADP by isolated mitochondria respiring on succinate was set by addition of ATP, ADP or ADP plus malonate. We measured the rates of phosphorylation and respiration and the protonmotive force under each of these conditions. We measured the oxygen consumption required to drive the proton leak at the protonmotive force reached under each condition and subtracted it from the respiration rate during phosphorylation to determine the oxygen consumption driving… CONTINUE READING
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