Effect of protein kinase C activators on the uptake of GABA by rat brain synaptosomes.

@article{Cupello1993EffectOP,
  title={Effect of protein kinase C activators on the uptake of GABA by rat brain synaptosomes.},
  author={Aroldo Cupello and Bruno Gasparetto and P. Cerruti Mainardi and Luigi Amedeo Vignolo and Mauro Robello},
  journal={The International journal of neuroscience},
  year={1993},
  volume={69 1-4},
  pages={
          131-6
        }
}
Synaptosomes were prepared from rat brain by a discontinuous Ficoll gradient method and used for studying the uptake of labelled GABA. Two GABA uptake components were evidenced, a high (Km = 3.13 microM) and a low (Km = 92.4 microM) affinity one. Preincubation of synaptosomes with two different activators of protein kinase C, phorbol 12, 13-diacetate (PDAc) and oleyl-acetyl glycerol (OAG), resulted in a change of GABA uptake. In particular, the low affinity component increased its Vmax by 58-74… CONTINUE READING