Effect of promoter‐leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells

@article{Ingersoll1996EffectOP,
  title={Effect of promoter‐leader sequences on transient expression of reporter gene chimeras biolistically transferred into sugarbeet (Beta vulgaris) suspension cells
},
  author={John C. Ingersoll and Thomas M. Heutte and Lowell D. Owens},
  journal={Plant Cell Reports},
  year={1996},
  volume={15},
  pages={836-840}
}
Summary. Chimeric constructs consisting of the gus coding region fused downstream of promoter‐untranslated leader sequences from the tobacco osmotin and PR‐S genes, the potato proteinase inhibitor 2 gene (pin2), and the cauliflower mosaic virus (CaMW) 35S promoter were biolistically transferred into sugarbeet suspension cells. Each construct was expressed in recipient cells at 6 h after bombardment with maximum levels observed between 12 and 48 h. Expression of the PR‐S construct mimicked the… CONTINUE READING

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