Effect of proline and arginine metabolism on freezing stress of Saccharomyces cerevisiae.

@article{Morita2002EffectOP,
  title={Effect of proline and arginine metabolism on freezing stress of Saccharomyces cerevisiae.},
  author={Yuko Morita and Shigeru Nakamori and Hiroshi Takagi},
  journal={Journal of bioscience and bioengineering},
  year={2002},
  volume={94 5},
  pages={
          390-4
        }
}
In Saccharomyces cerevisiae, the PUT1-encoded proline oxidase and the PUT2-encoded delta1-pyrroline-5-carboxylate dehydrogenase are required to convert proline to glutamate. We recently showed that a put1 disruptant accumulated higher levels of proline intracellularly and conferred higher resistance to freezing stress. Here, we determined the effect of put2 disruption on yeast cell viability under freezing stress. When grown on arginine as the sole nitrogen source, the put2 disruptant showed a… CONTINUE READING

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