Effect of primary culture medium type for culture of canine fibroblasts on production of cloned dogs.

Abstract

Fibroblasts are common source of donor cells for SCNT. It is suggested that donor cells' microenvironment, including the primary culture, affects development of reconstructed embryos. To prove this, canine embryos were cloned with fibroblasts that were cultured in two different primary media (RCMEp vs. Dulbecco's modified Eagle's medium [DMEM]) and in vivo… (More)
DOI: 10.1016/j.theriogenology.2015.04.007

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