Effect of positive inotropic agents on myosin isozyme population and mechanical activity of cultured rat heart myocytes.

@article{Rupp1991EffectOP,
  title={Effect of positive inotropic agents on myosin isozyme population and mechanical activity of cultured rat heart myocytes.},
  author={Heinz Rupp and Harvey J. Berger and Alexander Pfeifer and Karl Werdan},
  journal={Circulation research},
  year={1991},
  volume={68 4},
  pages={1164-73}
}
To examine whether catecholamines have a direct effect on myosin heavy chain expression of heart myocytes or whether they act via an altered work load, myocytes from neonatal rat hearts were cultured in thyroid hormone-free media containing various positive inotropic and chronotropic agents. The velocity and frequency of contraction of the myocytes were monitored using an optoelectronic system. After 3-5 days of culture, myosin isozyme populations, cellular cAMP content, and 2-deoxy-D-glucose… CONTINUE READING