Effect of plasmid backbone modification by different human CpG motifs on the immunogenicity of DNA vaccine vectors.

@article{Coban2005EffectOP,
  title={Effect of plasmid backbone modification by different human CpG motifs on the immunogenicity of DNA vaccine vectors.},
  author={Cevayir Coban and Ken J Ishii and Mayda Gursel and Dennis M Klinman and Nirbhay Kumar},
  journal={Journal of leukocyte biology},
  year={2005},
  volume={78 3},
  pages={647-55}
}
DNA vaccines, in general, have been found to be poorly immunogenic in nonhuman primates and humans as compared with mice. As the immunogenicity of DNA plasmids relies, to a large extent, on the presence of CpG motifs as built in adjuvants, we addressed the issue of poor immunogenicity by inserting recently identified CpG oligonucleotides (ODN) optimal for human (K-type or D-type CpG ODN) into the backbone of plasmid VR1020. We found that plasmid DNA containing K-type CpG motifs or D-type CpG… CONTINUE READING