Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro.

@article{Yadav2013EffectOP,
  title={Effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (Bubalus bubalis) embryos produced in vitro.},
  author={Anandprakash Yadav and Karn Pratap Singh and M K Singh and Neha Saini and Prabhat Palta and R. S. Manik and Suresh Kumar Singla and Ramesh Chandra Upadhyay and M. P. S. Chauhan},
  journal={Reproduction in domestic animals = Zuchthygiene},
  year={2013},
  volume={48 5},
  pages={858-65}
}
For investigating the effects of physiologically relevant heat shock, buffalo oocytes/embryos were cultured at 38.5°C (control) or were exposed to 39.5°C (Group II) or 40.5°C (Group III) for 2 h once every day throughout in vitro maturation (IVM), fertilization (IVF) and culture (IVC). Percentage of oocytes that developed to 8-cell, 16-cell or blastocyst stage was lower (p < 0.05) and the number of apoptotic nuclei was higher (p < 0.05) for Group III > Group II > controls. At both 8-16-cell and… CONTINUE READING