Effect of phosphorothioate modification of oligodeoxynucleotides on specific protein binding.

@article{Brown1994EffectOP,
  title={Effect of phosphorothioate modification of oligodeoxynucleotides on specific protein binding.},
  author={David Brown and Seung Hye Kang and Sergei M. Gryaznov and LAWRENCE A. DEDIONISIO and Otto Heidenreich and Stephen R. Sullivan and Xuejun Xu and M I Nerenberg},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 43},
  pages={26801-5}
}
Phosphorothioate modification of internucleoside linkages is widely used to prevent degradation of oligodeoxynucleotide (ODN) therapeutic agents in serum and cells. This modification generally increases ODN potency, but in many instances it is associated with an increase of poorly understood nonspecific effects. In this study, we have found that both cellular retention and nonspecific protein binding are dependent upon the extent of the oligonucleotide's modification. Flow cytometry of cells… CONTINUE READING
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