• Corpus ID: 8393297

Effect of phospholipid on aldosterone biosynthesis by a cytochrome P-450(11) beta-reconstituted system.

@article{Ohnishi1984EffectOP,
  title={Effect of phospholipid on aldosterone biosynthesis by a cytochrome P-450(11) beta-reconstituted system.},
  author={T. Ohnishi and Akira Wada and Yasuki Nonaka and Mitsuhiro Okamoto and Toshio Yamano},
  journal={Biochemistry international},
  year={1984},
  volume={9 6},
  pages={
          715-23
        }
}

• T. Ohnishi, A. Wada, +2 authors T. Yamano
• Published 1 December 1984
• Biology, Chemistry
• Biochemistry international

Effect of phospholipid on aldosterone synthesis catalyzed by a cytochrome P-450(11)beta-reconstituted system was examined. Corticosterone was incubated with P-450(11)beta in the presence of phosphatidylcholine, phosphatidylethanolamine, cardiolipin, phosphatidylinositol, or phosphatidylserine. These phospholipids stimulated the rate of aldosterone- and 18-hydroxycorticosterone-synthesis, although the mode of stimulation by neutral phospholipids was different from that by acidic phospholipids. In the presence of a phospholipid mixture containing the lipids in the same molar ratios as found in adrenocortical mitochondria, the rate of production of aldosterone increased 10-fold over the rate without the lipids, and that of 18-hydroxycorticosterone increased 3-fold. The maximal synthetic rates of aldosterone and 18-hydroxycorticosterone were 2 and 5 nmol/nmol P-450/min, respectively.