Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells

  title={Effect of phenylalanine on Taxol production and antioxidant activity of extracts of suspension-cultured hazel (Corylus avellana L.) cells},
  author={Ebrahim Bemani and Faezeh Ghanati and Ayatollah Rezaei and Mitra Jamshidi},
  journal={Journal of Natural Medicines},
Taxol is produced by a few microorganisms and plants such as yew (Taxus sp.). Recent researches have shown that hazel (Corylus avellana L.) is also able to produce Taxol. In the present study, effects of different concentrations of phenylalanine (Phe) on the production of Taxol, antioxidant activity, and cytotoxic effects of extracts of suspension-cultured hazel cells were investigated. The cells were treated with different concentrations of Phe on day 7 of subculture and were harvested on day… 

Aluminum elicitation improves antioxidant potential and taxol production in hazelnut (Corylus avellana L.) cell suspension culture

In general, for the increased taxol synthesis application of 50 and 100 μM of aluminium elicitor in cell culture provided acceptable results and aluminium improved its release into the medium and total yield in comparison to controls.

Change of antioxidant enzymes activity of hazel (Corylus avellana L.) cells by AgNPs

5 ppm AgNPs was the best concentration for elicitation of hazel cells to produce efficient amounts of H2O2 in order for stimulation of antioxidant defence system, production of Taxol at the highest capacity of the cells, meanwhile reserving their viability.

The influence of phenylalanine feeding on cell growth, antioxidant activity, phenylpropanoids content, and yield in cell suspension culture of Rhodiola imbricata (Edgew.)

The present work concluded that the precursor-mediated approach enhanced the overall phenylpropanoids production in the cell suspension culture of R. imbricata.

Viability-reducing activity of Coryllus avellana L. extracts against human cancer cell lines.

Rutin accumulation in gardenia calli cultures as a response to phenyl alanine and salicylic acid

BackgroundRutin is one of the flavonoid compounds found in different plant species. Rutin has powerful antioxidant properties. The main target of this study is to elevate rutin content in gardenia

Factors affecting the growth, antioxidant potential, and secondary metabolites production in hazel callus cultures

Hazelnut is one of the most important nut plants recently suggested as a sustainable source for paclitaxel. In the present study, the effect of the concentration and combination of PGRs, different

Fine quantitation of novel trace taxans in suspension-cultured Corylus avellana L. cells by photo diode array HPLC technique.

The results presented here can provide approaches in decision making and time management toward extract maximum amounts of a desired taxan in suspension-cultured hazel cells.

Effects of Storage Time and Temperature on Antioxidants in Juice from Momordica charantia L. and Momordica charantia L. var. abbreviata Ser.

The results revealed that the antioxidant activity ofMCVAS was better than that of MC, possibly because of richer components of MCVAS, and the effects of storage time and storage temperature on these antioxidant activities were investigated.



Enhancement of taxol production and release in Taxus chinensis cell cultures by ultrasound, methyl jasmonate and in situ solvent extraction

  • J. WuL. Lin
  • Biology
    Applied Microbiology and Biotechnology
  • 2003
It was found that the organic solvent DBP, as well as US and MJ, stimulated the enzyme activity of secondary metabolic pathways, which was partially responsible for the enhanced taxol production.

Improved Growth and Taxol Yield in Developing Calli of Taxus cuspidata by Medium Composition Modification

Analysis of the effect of different culture media components on growth and production of taxol in developing callus cultures of T. cuspidata found significant increases in taxol yield and growth over basal medium grown calli were observed with some of the modified media.

Production of Taxol and its Analogues from Cell Cultures of Taxus wallichiana

Preursors and growth retardants significantly improved the production of taxol, DAB and BA in cultures grown in production medium and on HPLC analysis using photodiode array detector.

Improved taxol yield by aromatic carboxylic acid and amino acid feeding to cell cultures of taxus cuspidata

Examination of the effect of various concentrations of phenylalanine, benzoic acid, N‐benzoylglycine, serine, glycine, alanine, and 3‐amino‐3‐phenyl‐propionic acid on taxol accumulation in cell suspensions of Taxus cuspidata, cell line FCL1F, and in developing callus cultures finds increases in taxol amounts at stationary phase brought by the most effective precursors.

Acting Point in Taxol Biosynthesis Pathway of Elicitor in Suspension Cultures of Taxus chinensis var. mairei

There are three possibilities for salicylic acid and arachidonic acid: improving the biosynthesis of 10-deactyl baccatin III (10-DAB), inhibiting the degradation of taxol and inhibition of cephalomannine but it is difficult to elucidate the exact acting paths of these two elicitors.

In vitro cell cultures obtained from different explants of Corylus avellana produce Taxol and taxanes

Hazel cell cultures produce Taxol and taxanes under controlled conditions, suggesting that hazel possesses the enzymes for Taxol production, which until now was considered to be a pathway particular to Taxus genus.

Antioxidant activity of extracts from Acacia confusa bark and heartwood.

The results indicate that both bark and heartwood extracts clearly have strong antioxidant effects, and the heartwood extract was more effective than the bark extract for the superoxide radical scavenging activity.