Effect of patient, operative and isolation factors on subsequent yield and viability of human hepatocytes for research use

@article{Lloyd2004EffectOP,
  title={Effect of patient, operative and isolation factors on subsequent yield and viability of human hepatocytes for research use},
  author={T.D.R. Lloyd and Samantha Orr and Robin Patel and G. Crees and Satyajeetsingh Chavda and H. Vadyar and David Paul Berry and David Sherlock and Ashley Robert Dennison},
  journal={Cell and Tissue Banking},
  year={2004},
  volume={5},
  pages={81-87}
}
It is widely accepted that the model of choice for pharmacotoxicological studies are human hepatocytes. There is therefore a demand for these cells, but quality must be maintained for their widespread use. We present a retrospective review of the isolation of hepatocytes from both surgically resected tissue and livers rejected for transplantation, and evaluated patient, operative and isolation variables to ascertain which may affect the viability and yield of cells. Seven clinically rejected… CONTINUE READING

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