Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas.

@article{Ruiz2017EffectOO,
  title={Effect of oocyte maturation time, sperm selection method and oxygen tension on in vitro embryo development in alpacas.},
  author={Jaime Jesus Ruiz and R Paulo Santayana and M Jos{\'e} Mendoza and J Leandra Landeo and Elizabeth Huam{\'a}n and Flamel Ticllacuri and L Fidel Mujica and Mauricio R. Silva and M H Ratto},
  journal={Theriogenology},
  year={2017},
  volume={95},
  pages={127-132}
}
We evaluated the effect of in vitro maturation time, sperm selection and oxygen tension on alpaca embryo development. In Experiment I, Cumulus Oocyte- Complexes (COCs) were obtained from abattoir ovaries and in vitro matured in TCM-199 for 24 (n = 217), 28 (215), or 32 h (223) at 38.5 °C, high humidity and 5% CO2 in air. Oocytes from 24 (n = 392), 28 (n = 456) or 32 (n = 368) h groups were in vitro fertilized with epididymal sperm and cultured in SOFaa at 38.5 °C, high humidity and 5% CO2, 5… CONTINUE READING

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