Effect of nutritional status on the fatty acid composition of rat liver and cultured hepatocytes

  title={Effect of nutritional status on the fatty acid composition of rat liver and cultured hepatocytes},
  author={Gary J. Nelson and Darshan S. Kelley and James M E Hunt},
The lipid concentration and fatty acid composition of the whole liver and of cultured hepatocytes isolated from the livers of rats fed ad libitum (fed), fasted for 24 hr (fasted), or fasted for 48 hr and then refed a fat-free, high carbohydrate diet for 48 hr (refed) was studied. Hepatocytes were maintained as monolayer cultures in serum-free, lipid-free media and their fatty acid composition was analyzed at 3, 24, 48, 72 and 96 hr. The livers of fed animals, as well as their hepatocytes… CONTINUE READING


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