Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and imidazole complexes.

@article{Berry2009EffectOM,
  title={Effect of mutation of carboxyl side-chain amino acids near the heme on the midpoint potentials and ligand binding constants of nitrophorin 2 and its NO, histamine, and imidazole complexes.},
  author={Robert E. Berry and Maxim Nikolaievich Shokhirev and Arthur Y W Ho and Fei Yang and Tatiana Kh Shokhireva and Hongjun Zhang and Andrzej Weichsel and William R. Montfort and Francis A. Walker},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 6},
  pages={2313-27}
}
Nitrophorins (NPs) are a group of NO-carrying heme proteins found in the saliva of a blood-sucking insect from tropical Central and South America, Rhodnius prolixus, the "kissing bug". NO is kept stable for long periods of time by binding it as an axial ligand to a ferriheme center. The fact that the nitrophorins are stabilized as Fe(III)-NO proteins is a unique property because most heme proteins are readily autoreduced by excess NO and bind NO to the Fe(II) heme irreversibly (K(d)s in the… CONTINUE READING