Effect of modified MRS medium on production and purification of antimicrobial peptide ST4SA produced by Enterococcus mundtii.

@article{Todorov2009EffectOM,
  title={Effect of modified MRS medium on production and purification of antimicrobial peptide ST4SA produced by Enterococcus mundtii.},
  author={Svetoslav Dimitrov Todorov and Leon Milner Theodore Dicks},
  journal={Anaerobe},
  year={2009},
  volume={15 3},
  pages={65-73}
}
Highest antimicrobial activity of peptide ST4SA (51,200 AU/mL) was recorded after 14 h of growth in MRS broth with optimal production at pH 6.0 or 6.5. Growth of strain ST4SA in the presence of tryptone, yeast extract, or a combination of the two, yielded 102,400 AU/mL. An increase in production of peptide ST4SA to 102,400 AU/mL was recorded in the presence of 20.0 g/L fructose, but decreased to 25,600 AU/mL in the presence of lactose (20.0 g/L) or mannose (20.0 g/L) as sole carbon source… CONTINUE READING
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