Effect of mild-thiol reducing agents and alpha2,3-sialyltransferase expression on secretion and sialylation of recombinant EPO in CHO cells.

@article{Chang2013EffectOM,
  title={Effect of mild-thiol reducing agents and alpha2,3-sialyltransferase expression on secretion and sialylation of recombinant EPO in CHO cells.},
  author={Kern Hee Chang and Yeon Tae Jeong and Chan Yeong Kwak and One Kyun Choi and Jung Kim},
  journal={Journal of microbiology and biotechnology},
  year={2013},
  volume={23 5},
  pages={699-706}
}
We have previously reported that N-acetylcysteine (NAC) not only delayed apoptosis but also enhanced the production of recombinant erythropoietin (EPO) in Chinese hamster ovary (CHO) cell culture. To investigate the production enhancement mechanism, the effects of similar thiolreducing agents were studied. Intriguingly, all mild reducing agents examined… CONTINUE READING