Effect of microinjection time during postfertilization S-phase on bovine embryonic development.

@article{Gagn1995EffectOM,
  title={Effect of microinjection time during postfertilization S-phase on bovine embryonic development.},
  author={M B Gagn{\'e} and François Pothier and Marc-Andr{\'e} Sirard},
  journal={Molecular reproduction and development},
  year={1995},
  volume={41 2},
  pages={184-94}
}
Microinjection into bovine zygotes was performed to evaluate the effects of the timing of injection during the phase of DNA replication on the subsequent in vitro development of embryos and expression of injected chicken beta-actin promoter-lac Z gene construct. The period of DNA replication of bovine zygotes, determined by 3H-thymidine incorporation, begins between 12 hr and 13 hr postinsemination (hpi) of in vitro matured oocytes, reaches a maximum from 17 hpi to 19 hpi, and is complete by 21… CONTINUE READING