Effect of methotrexate (MTX) on NAD(P)+ dehydrogenases of HeLa cells: malic enzyme, 2‐oxoglutarate and isocitrate dehydrogenases

@article{Caetano1997EffectOM,
  title={Effect of methotrexate (MTX) on NAD(P)+ dehydrogenases of HeLa cells: malic enzyme, 2‐oxoglutarate and isocitrate dehydrogenases},
  author={N. N Caetano and Annibal P. Campello and Eva Gunilla Skare Carnieri and Ma. L{\'u}cia W. Kluppel and M B M Oliveira},
  journal={Cell Biochemistry and Function},
  year={1997},
  volume={15}
}
The effects of methotrexate (MTX) on oxygen uptake by permeabilized HeLa cells were evaluated. MTX did not inhibit state III respiration when the oxidizable substrate was succinate, but when the substrates were 2‐oxoglutarate or isocitrate the respiration decreased about 50 per cent at 1·0 mM concentration of the drug. This effect was explained by inhibition of 2‐oxoglutarate and isocitrate dehydrogenases by MTX. No effect was observed on succinate dehydrogenase. An evaluation of the effects of… Expand
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