Effect of methionine deprivation on L5178Y murine leukemia cells in culture interference with the antineoplastic effect of methotrexate.

@article{Chello1976EffectOM,
  title={Effect of methionine deprivation on L5178Y murine leukemia cells in culture interference with the antineoplastic effect of methotrexate.},
  author={P. Chello and J. Bertino},
  journal={Biochemical pharmacology},
  year={1976},
  volume={25 8},
  pages={
          889-92
        }
}
  • P. Chello, J. Bertino
  • Published 1976
  • Chemistry, Medicine
  • Biochemical pharmacology
  • Abstract L5178Y cells in culture have a requirement for l -methionine which cannot be satisfied by supplying the components necessary for de novo biosynthesis [1]. Methionine deprivation produced a rapid and progressive loss of cell viability (30°o by 6 hr: 90°o by 24 hr). Cells which remained viable after being deprived of methionine could be rescued by l -methionine supplementation. L5178Y cells in culture were also highly sensitive to the folate antagonist, methotrexate. Exposure to a… CONTINUE READING
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    References

    SHOWING 1-10 OF 14 REFERENCES
    Purineless death as a link between growth rate and cytotoxicity by methotrexate.
    • 81
    • PDF
    N5-methyltetrahydrofolate: homocysteine methyltransferase activity in extracts from normal, malignant and embryonic tissue culture cells.
    • 62
    The mechanism of action of methotrexate in cultured L5178Y leukemia cells.
    • 72