Effect of membrane potential on the kinetic parameters of the Na+ or H+ melibiose symport in Escherichia coli membrane vesicles.

@article{Bassilana1985EffectOM,
  title={Effect of membrane potential on the kinetic parameters of the Na+ or H+ melibiose symport in Escherichia coli membrane vesicles.},
  author={Martine Bassilana and E Damiano-Forano and G{\'e}rard Leblanc},
  journal={Biochemical and biophysical research communications},
  year={1985},
  volume={129 3},
  pages={
          626-31
        }
}
Comparison of the transport properties of the melibiose permease of E. coli acting as a H+-symport or a Na+-symport has been performed by measuring initial rates of [3H]-melibiose transport or its accumulation in isolated membrane vesicles. The results show strikingly that although the membrane potential primarily drives melibiose accumulation by both types of symport, it selectively affects the apparent affinity constant Kt of the H+-melibiose symport while it specifically changes the maximal… CONTINUE READING
BETA