Effect of lipo-prostaglandin E1 on mesangial proliferative glomerulonephritis in rats

Abstract

The antinephritic effect of lipo-prostaglandin E1, prostaglandin E1 incorporated in lipid microspheres was investigated using an experimental model of mesangial proliferative glomerulonephritis (MsPGN). Twenty-two female rats were randomly divided into nephritic group (N,n=6), lipo-prostaglandin E1 treated group (NL,n=8) and control group (C,n=6). Lipo-prostaglandin E1 was given intravenously at 40 μg·kg−1·d−1 from the 6th week to the 8th week. Twenty-four h urinary protein contents and blood creatinine (Cr) were determined and the pathological changes were observed in the experiment. The expression of proliferating cell nuclear antigen (PCNA), extracellular matrix (fibronectin, FN; collagen type IV, Col IV) and transforming growth factorβ 1 (TGFβ 1) was detected by using immunohistochemistry. The results showed that lipo-prostaglandin E1 significantly inhibited the glomerular histopathological changes as well as the elevation of plasma Cr (P<0.05). The overexpression of PCNA, FN, Col IV and TGFβ 1 were also obviously inhibited in group NL as compared with the group N (P<0.01). It was suggested that lipo-prostaglandin E1 could improve renal function, inhibit the proliferation of glomerular cells and reduce the deposition of extracellular matrix, which may be related to the down-regulation of the TGFβ 1 expression.

DOI: 10.1007/BF02896004

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Cite this paper

@article{Changqing2005EffectOL, title={Effect of lipo-prostaglandin E1 on mesangial proliferative glomerulonephritis in rats}, author={Luo Changqing and Zhang Xiaoli and Wang Yumei and Zhu Zhonghua and Deng Anguo}, journal={Journal of Huazhong University of Science and Technology [Medical Sciences]}, year={2005}, volume={25}, pages={516-518} }