Effect of laser microbeam irradiation of the nucleus on the cleavage of mouse eggs in culture.

Abstract

Two-cell mouse eggs were irradiated by a helium-cadmium laser on a spot of about 4 micron2 (d = 2.2 micron) in one or both nuclei either continuously or repeatedly at 0.36 erg micron-2 sec-1 and then cultured to observe cellular development. After exposing one nucleus to the microbeam to five or seven 1-sec pulses (1.80 or 2.52 ergs micron-2, respectively… (More)

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