Effect of juvenile hormone on the embryogenesis of a polyembryonic wasp, copidosoma floridanum, in vitro

  title={Effect of juvenile hormone on the embryogenesis of a polyembryonic wasp, copidosoma floridanum, in vitro},
  author={Kikuo Iwabuchi},
  journal={In Vitro Cellular \& Developmental Biology - Animal},
  • K. Iwabuchi
  • Published 1 November 1995
  • Biology
  • In Vitro Cellular & Developmental Biology - Animal
SummarySingle two-cell-stage embryos of a polyembryonic waspCopidosoma floridanum cultured in 20 µl droplets of culture medium developed to morulae at the same developmental rate as those in host eggs, but the subsequent development into polymorulae was inferior. This inferior development was markedly improved by addition of juvenile hormone (I, II, or III) or its analogues to the culture medium in a concentration-dependent manner. 

Host–Polyembryonic Parasitoid Interactions

The polyembryonic egg–larval endoparasitoid Copidosoma floridanum cannot employ this strategy due to its prolonged morula stage, so this species has evolved a novel approach for entering the host body that involves tissue-compatible invasion by the motile morula.

Compatible invasion of a phylogenetically distant host embryo by a hymenopteran parasitoid embryo

It is shown that the morula-stage embryo of C. floridanum actively invades the host embryo, providing a novel case of embryonic invasion into a phylogenetically distant host embryos, ensuring cellular compatibility with host tissues.

Gene Expression of Protein-Coding and Non-Coding RNAs Related to Polyembryogenesis in the Parasitic Wasp, Copidosoma floridanum

Polyembryony is a unique form of development in which many embryos are clonally produced from a single egg. Polyembryony is known to occur in many animals, but the underlying genetic mechanism

Analysis of molecular mechanism for acceleration of polyembryony using gene functional annotation pipeline in Copidosoma floridanum

Gene enrichment analysis revealed genes associated with platelet degranulation, fatty acid biosynthesis, cell morphogenesis in the differentiation and integrin signaling pathways were fluctuated following JH treatment, and Cytoscape analysis revealed a molecular interaction that was possibly associated with polyembryogenesis.



Ecdysteroid induction of embryonic morphogenesis in a parasitic wasp.

It is reported that C. floridanum embryos must develop for 9 days before acquiring the competence to undergo morphogenesis, and several pieces of evidence suggest that ecdysteroids of host origin regulate induction of C. Floridanum embryonic morphogenesis.

Laboratory rearing method for Acanthoplusia agnata (Lepidoptera: Noctuidae) without change of artifical diet.

A simple rearing method for Acanthoplusia agnata which does not require the change of artificial diet was developed and a higher ratio of pupation was attained when the insects were transferred to the cup containing the artificial diet at the 2nd or 3rd instar larval stages.

Qualitative and quantitative analyses of juvenile hormone and ecdysteroids from the egg to the pupal molt in Trichoplusia ni.

A method was developed to determine in the same extract juvenile hormone and various types of ecdysteroids in precisely staged eggs and larvae of Trichoplusia ni, whereas in larval stages juvenile hormone II appeared to be the predominant or exclusive juvenile hormone.

Endocrine interrelationship between the parasitoid Chelonus sp. and its host Trichoplusia ni.

A comparison revealed that both ecdysteroids and juvenile hormone fluctuate independently in parasitoid and host at most stages, suggesting that the parasitoids produces its own hormones.

9 – Pharmacology of Insect Juvenile Hormones

The occurrence of a juvenile hormone binding protein and in vitro synthesis of juvenile hormone by the serosa of Locusta migratoria embryos

Serosal epitheliae of 4- to 5-day-old embryos contain an immunohistologically detectable cytosolic protein which is related to the juvenile hormone carrier-protein in the haemolymph and which binds tritiated juvenile hormone 3 (JH3) in organ cultures, and represent a hitherto unrecognized source of methyl transferase activity and of JH3 production.