Effect of introducing proline residues on the stability of Aspergillus awamori.

@article{Li1997EffectOI,
  title={Effect of introducing proline residues on the stability of Aspergillus awamori.},
  author={Yuan Feng Li and Peter J. Reilly and Clark Ford},
  journal={Protein engineering},
  year={1997},
  volume={10 10},
  pages={1199-204}
}
In Aspergillus awamori glucoamylase, Ala27, Ala393, Ala435, Ser436 and Ser460 were replaced with proline residues, in order to stabilize the enzyme by forming more rigid peptide backbones. Specific activities were unaffected except for a decrease in Ser460-->Pro glucoamylase. Thermostability was increased in Ser436-->Pro glucoamylase, unchanged in Ala435… CONTINUE READING