Effect of interleukin 1 on the induction and maintenance of B cell tolerance in vitro.


The effects of interleukin 1 (IL-1) on induction and maintenance of immune tolerance in vitro have been studied by using splenocytes from C57BL/6 male mice. B cell tolerance to the hapten trinitrophenol (TNP) was induced with TNP-human gamma globulin (HGG) and the cells were challenged with TNP-Ficoll. To determine the tolerance (or immunity), antibody concentrations in the supernatant fluids were measured by using a TNP-specific ELISA assay. Partially purified murine IL-1 abrogated tolerance induction, and when it was added at challenge phase, it also abrogated tolerance. In addition, partially purified IL-1 converted TNP-HGG from a tolerogen into an immunogen without any additional exposure to antigen. Similar results were obtained when recombinant human IL-1 alpha was used in place of partially purified natural IL-1. IL-1 is most likely acting directly on B cells rather than through the agency of T cells because purified B cells failed to become tolerant in the presence of IL-1. Studies of IL-1 production by antigen- or tolerogen-stimulated splenocytes or purified B cells showed that only antigen could elicit IL-1 production in these cells. That tolerance abrogation is unique to IL-1 is suggested by studies which show that TNF, IL-2, and INF gamma, alone, in combination with each other, or in combination with subeffective concentrations of IL-1 failed to effect tolerance abrogation.

Cite this paper

@article{Duan1989EffectOI, title={Effect of interleukin 1 on the induction and maintenance of B cell tolerance in vitro.}, author={Jingwu Duan and Gail S Habicht}, journal={Journal of leukocyte biology}, year={1989}, volume={45 4}, pages={329-35} }