Effect of interleukin 1 beta on osteoblastic clone MC3T3-E1 cells

@article{Ikeda1988EffectOI,
  title={Effect of interleukin 1 beta on osteoblastic clone MC3T3-E1 cells},
  author={Dr. Eiko Ikeda and Masami Kusaka and Yoshiyuki Hakeda and Kazushige Yokota and Masayoshi Kumegawa and Shozo Yamamoto},
  journal={Calcified Tissue International},
  year={1988},
  volume={43},
  pages={162-166}
}
The effect of recombinant interleukin 1 Beta (IL-1(β)) was investigated on osteoblastic cell line MC3T3-E1 cloned from mouse calvaria. IL-1(β) stimulated cell proliferation which increased cell number and caused dose-related stimulation of DNA synthesis, with a maximal effect at a concentration of 12.5 U/ml; suppressed alkaline phosphatase activity and collagen synthesis maximally at 0.5 and 62.5 U/ml, respectively; and increased the amount of free [3H] hydroxyproline in the cultures, but the… CONTINUE READING