Effect of interactions between granulosa and thecal cells on meiotic arrest in bovine oocytes.

@article{Kotsuji1994EffectOI,
  title={Effect of interactions between granulosa and thecal cells on meiotic arrest in bovine oocytes.},
  author={Fumikazu Kotsuji and Mitsumasa Kubo and Tan Tominaga},
  journal={Journal of reproduction and fertility},
  year={1994},
  volume={100 1},
  pages={151-6}
}
An in vitro culture system in which two types of cell from ovarian follicular walls were attached to opposite surfaces of a collagen membrane was developed. The effect of granulosa-theca cell communication on oocyte maturation was investigated using this culture system. Bovine cumulus-oocyte complexes were incubated with granulosa cells, with or without theca cells. The time course of germinal vesicle breakdown was assessed. When cultured with granulosa cells alone, oocytes showed 100% germinal… CONTINUE READING

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