Effect of inorganic phosphate on the force and number of myosin cross-bridges during the isometric contraction of permeabilized muscle fibers from rabbit psoas.

@article{Caremani2008EffectOI,
  title={Effect of inorganic phosphate on the force and number of myosin cross-bridges during the isometric contraction of permeabilized muscle fibers from rabbit psoas.},
  author={Marco Caremani and Jody A. Dantzig and Yale E. Goldman and Vincenzo Lombardi and Marco Linari},
  journal={Biophysical journal},
  year={2008},
  volume={95 12},
  pages={5798-808}
}
The relation between the chemical and mechanical steps of the myosin-actin ATPase reaction that leads to generation of isometric force in fast skeletal muscle was investigated in demembranated fibers of rabbit psoas muscle by determining the effect of the concentration of inorganic phosphate (Pi) on the stiffness of the half-sarcomere (hs) during transient and steady-state conditions of the isometric contraction (temperature 12 degrees C, sarcomere length 2.5 mum). Changes in the hs strain were… CONTINUE READING