Effect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799.

@article{Coln1995EffectOI,
  title={Effect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799.},
  author={Grace Eileen Col{\'o}n and Mike S. M. Jetten and Thank Thuong Nguyen and M. E. Gubler and Maximillian T. Follettie and Anthony J Sinskey and Gregory Stephanopoulos},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 1},
  pages={
          74-8
        }
}
Amplification of the operon homdr-thrB encoding a feedback-insensitive homoserine dehydrogenase and a wild-type homoserine kinase in a Corynebacterium lactofermentum lysine-producing strain resulted in both homoserine and threonine accumulation, with some residual lysine production. A plasmid enabling separate transcriptional control of each gene was constructed to determine the effect of various enzyme activity ratios on metabolite accumulation. By increasing the activity of homoserine kinase… CONTINUE READING

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