Effect of incubation media on the recovery of Escherichia coli K12 heated at 52 degrees C.

@article{Ahmad1978EffectOI,
  title={Effect of incubation media on the recovery of Escherichia coli K12 heated at 52 degrees C.},
  author={M. Ghufran Ahmad and Brahm S. Srivastava and Seema Agarwala},
  journal={Journal of general microbiology},
  year={1978},
  volume={107 1},
  pages={37-44}
}
The exposure of exponentially grown Escherichia coli K12 to 52 degrees C for 30 min in Tris/Mg2+ buffer resulted in a considerable loss of viability when plated on tryptone agar. When such heated bacteria were held at 37 degrees C for 2 h in tryptone broth before plating on tryptone agar, there was a significant increase in viability. Thus, heat damage was repaired in tryptone broth but not on tryptone agar. Recovery was greater in tryptone broth than in synthetic medium. In tryptone broth… CONTINUE READING

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