Effect of including growth factors and antioxidants in maturation medium used for in vitro culture of buffalo oocytes recovered in vivo.

@article{Singhal2009EffectOI,
  title={Effect of including growth factors and antioxidants in maturation medium used for in vitro culture of buffalo oocytes recovered in vivo.},
  author={Sumit Singhal and Shiv Charan Prasad and Beerendra Singh and Jai Kishan Prasad and Harihar Prasad Gupta},
  journal={Animal reproduction science},
  year={2009},
  volume={113 1-4},
  pages={44-50}
}
This study examined the effect of including one of two growth factors (100 ng/ml IGF-1 or 20 ng/ml EGF) in combination with one of two antioxidants (50 microM cysteamine or 50 microM beta-mercaptoethanol) in maturation, fertilization and subsequent development of buffalo oocytes. The oocytes were recovered by in vivo ovum pick-up technique from six Murrah buffalo heifers twice a week over a period of 16 weeks. Immediately after ovum pick-up oocytes recovered from six donors were allocated… CONTINUE READING
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