Effect of in vitro storage at 4 °C on survival and proliferation of poplar shoots

  title={Effect of in vitro storage at 4 °C on survival and proliferation of poplar shoots},
  author={J. Hausman and Olivier Neys and C. Kevers and T. Gaspar},
  journal={Plant Cell, Tissue and Organ Culture},
  • J. Hausman, Olivier Neys, +1 author T. Gaspar
  • Published 2004
  • Biology
  • Plant Cell, Tissue and Organ Culture
  • Shoot explants of in vitro proliferating cultures of Populus tremula (L.) x Populus tremuloides (L.) were stored for three months at 4°C, in dark or light, in basal culture medium with or without 2-isopentenyladenine (2iP), and in rooting medium with naphthalene acetic acid. They were transferred to cold at different times after subculturing. One hundred percent of shoots survived all tested conditions, in spite of leaf browing and necrosis. After transfer to 24°C for 2 weeks and a normal… CONTINUE READING
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