Effect of imidazole derivatives on cytochrome P-450 enzyme activities in a reconstructed human epidermis.

@article{Cotovio1996EffectOI,
  title={Effect of imidazole derivatives on cytochrome P-450 enzyme activities in a reconstructed human epidermis.},
  author={Jos{\'e} Cotovio and Roland Roguet and F X Pion and A Rougier and Jacques Leclaire},
  journal={Skin pharmacology : the official journal of the Skin Pharmacology Society},
  year={1996},
  volume={9 4},
  pages={242-9}
}
We tested the effect of various imidazole derivatives applied topically, on P-450-dependent enzyme activity of a reconstructed epidermis in conditions simulating clinical use. At nontoxic concentrations (determined by a cytotoxicity test based on the reduction of a tetrazolium salt, MTT, by mitochondrial deshydrogenase) econazole and clotrimazole had a biphasic effect on 7-ethoxycoumarin-O-deethylase (ECOD) activity in the epidermis, with induction at low concentrations and inhibition at high… CONTINUE READING