Effect of hypoxic conditions on in vitro susceptibility testing of amphotericin B, itraconazole and micafungin against Aspergillus and Candida.

@article{Warn2004EffectOH,
  title={Effect of hypoxic conditions on in vitro susceptibility testing of amphotericin B, itraconazole and micafungin against Aspergillus and Candida.},
  author={Peter A. Warn and Andrew Sharp and Jes{\'u}s Guinea and David William Denning},
  journal={The Journal of antimicrobial chemotherapy},
  year={2004},
  volume={53 5},
  pages={743-9}
}
OBJECTIVES The aim of this study was to investigate the effect of hypoxic conditions on in vitro susceptibility testing of amphotericin B, itraconazole and micafungin against Aspergillus (four species) and Candida (six species). METHODS In vitro susceptibility tests were set up according to NCCLS M27-A2 and M38-A recommendations, but incubation atmospheres were either air plus 5% CO(2), 1% oxygen/5% CO(2)/94% nitrogen or 0.25% oxygen/5% CO(2)/94.75% nitrogen. RESULTS In all Aspergillus… CONTINUE READING