Effect of hypoxia on the cytotoxicity of misonidazole in HELA S3 cells in vitro.

  title={Effect of hypoxia on the cytotoxicity of misonidazole in HELA S3 cells in vitro.},
  author={Y. Ohizumi and C. Shibata and T. Mori},
  volume={71 3},
A simple method to establish hypoxic conditions in vitro is described and the cytotoxicity and radiation dose-modifying effect of misonidazole were investigated; using HeLa S3 cells. Under hypoxic conditions, 5-hr exposure to 50mM misonidazole resulted in severe cytotoxicity, though the toxicity at 1mM was low. The survival curves of cells exposed to 5 or 10mM misonidazole showed an initial exponential portion followed by a less steep decline. Under aerobic conditions, exposure to 1mM for 5… Expand
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