Effect of hydration on interstitial distribution of charged albumin in rat dermis in vitro.

@article{Wiig2005EffectOH,
  title={Effect of hydration on interstitial distribution of charged albumin in rat dermis in vitro.},
  author={Helge Wiig and Olav Tenstad and Joel L. Bert},
  journal={The Journal of physiology},
  year={2005},
  volume={569 Pt 2},
  pages={631-41}
}
At physiological pH, negatively charged glycosaminoglycans in the extracellular matrix may influence distribution volume of macromolecular probes, a phenomenon of importance for hydration of the interstitium and therefore for body fluid balance. We hypothesized that such charge effect was dependent on hydration. Human serum albumin (HSA) (the pH value for the isoelectric point (pI) = 4.9) was made neutral by cationization (cHSA) (pI = 7.6). Rat dermis was studied in vitro in a specially… CONTINUE READING