Effect of heptaminol AMP amidate, a new nucleotide derivative, on in vitro humoral immunity.

  title={Effect of heptaminol AMP amidate, a new nucleotide derivative, on in vitro humoral immunity.},
  author={A. Saha and K. Ueno and S. Ohmori and T. Igarashi and H. Kitagawa},
  journal={Japanese journal of pharmacology},
  volume={47 1},
Heptaminol AMP amidate (HAA), a newly developed derivative of 5'-AMP, was found to potentiate the in vitro primary humoral immune response against T cell-dependent antigen, sheep red blood cells, when HAA was present in the early phase of spleen cell culture. Such a potentiating effect was not found against T cell-independent antigens such as lipopolysaccharide (LPS), trinitrophenylated (TNP)-LPS and TNP-Ficoll. The pattern of HAA-mediated immunopotentiation was similar to that of dibutyryl… Expand
2 Citations
Effects of heptaminol AMP amidate on suppressor and helper function of murine T cells.
The present results suggested that HAA-mediated immunopotentiation was possible by a combined suppressive effect on Ts cells and enhancing effect on Th cells. Expand
Effects of heptaminol AMP amidate (HAA) on cyclic AMP level and mitogen-induced proliferation of murine spleen cells.
The present results supported the previous findings that HAA-mediated immunopotentiation was closely related with a cAMP level elevating property of HAA, and the compound also enhanced the function of helper T cells. Expand


Immunopotentiating activity of a nucleotide derivative, heptaminol AMP amidate (HAA) in mice and spontaneously hypertensive rats (SHR).
In spontaneously hypertensive rats, with a state of immunosuppression, 10 days consecutive administrations of HAA at the dose of 10 mg/kg was found to increase significantly the anti-SRBC PFC and antibody titer values. Expand
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Lipopolysaccharide-induced activation of suppressor cells: reversal by an agent which alters cyclic nucleotide metabolism.
The effects of bacterial lipopolysaccharides (LPS) on the suppressor activity of mouse spleen cells were examined. Pretreatment with LPS was shown to induce suppressor activity in vivo and in vitro.Expand
Regulation of immune responses. I. Effects of cyclic AMP and cyclic GMP on immune induction.
agents that raise intracellular cAMP levels increase the magnitude of an in vitro primary humoral immune response when added at 10 −3 M during the first 12 hr of a 108 hr culture. Expand
Cyclic AMP and immune responses. I. Influence of poly A:U and cAMP on antibody formation in vitro.
The modification of antibody formation in vitro can be attributed to an alteration of cyclic AMP-mediated events, particularly since cAMP itself as well as the supplementation of poly A:U with theophylline can increase the number of antibody-forming cells that can be activated in vitro in the presence of a given amount of antigen. Expand
Activation of human B lymphocytes VII. the regulatory effect of cyclic adenosine monophosphate on human B cell activation.
  • P. Katz, A. Fauci
  • Biology, Medicine
  • The Journal of allergy and clinical immunology
  • 1978
It was demonstrated that dibutyryl cyclic adenosine monophosphate (DB-cAMP) markedly increased the PFC response of pokeweed mitogen--stimulated lymphocytes, and the mechanism of action is most likely a relative increase in helper T cell function resulting from a selective inhibition of suppressor T cells. Expand
Augmentation of the in vitro humoral immune response by pharmacologic agents. I: An explanation for the differential enhancement of humoral immunity via agents that elevate cAMP.
Results suggest that cAMP may be an important immunoregulatory signal, and that a variety of pharmacologic agents that modulate the induction of the humoral immune response may operate via this as a final common biochemical pathway. Expand
cAMP is an essential signal in the induction of antibody production by B cells but inhibits helper function of T cells.
The findings indicate that cAMP blocks the production of an inhibitor of BCGF activity, and it is proposed that IL 1 and an elevation in cytoplasmic cAMP represent minimal signal requirements for B cell activation. Expand
Regulatory substances produced by lymphocytes. VIII. Cell cycle specificity of inhibitor of DNA synthesis (IDS) action in lymphocytes.
The findings suggest that the cell cycle specificity of IDS may be due to appearance of cell receptors for IDS only during a limited phase of the lymphocyte cell cycle in mature cells, and that this cell cycle Specificity may be an important factor regulating IDS activity. Expand
Characterization of a specific adenosine receptor on human lymphocytes.
The results suggest that adenosine increases the intracellular cAMP content of lymphocytes as a result of its interaction with a specific membrane receptor which results in the activation of adenylate cyclase. Expand